eco ri sites (TaKaRa)
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eco ri sites
Eco Ri Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eco+ri+sites/EcoR+I/pmc13185574-204-41-46
Average 99 stars, based on 16033 article reviews
Eco Ri Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 16033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eco+ri+sites/EcoR+I/pmc13185574-204-41-46
Average 99 stars, based on 16033 article reviews
eco ri sites - by Bioz Stars,
2026-10
99/100 stars
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Produced:Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation. Article Snippet: .. GFPFis1 was produced by PCR- based cloning of rat Fis1 [amplified from p3xFLAG- CMV- 10- Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation Article Snippet: .. GFP-Fis1 was produced by PCR-based cloning of rat Fis1 [amplified from p3xFLAG-CMV-10-Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Polymerase Chain Reaction:Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation. Article Snippet: .. GFPFis1 was produced by PCR- based cloning of rat Fis1 [amplified from p3xFLAG- CMV- 10- Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: Engineering back-splicing-like sites for enhanced linear RNA expression Article Snippet: .. The recovered PCR products were connected with the linear transfer vector pFastBacdual at the Xba I and Article Title: Engineering back-splicing-like sites for enhanced linear RNA expression Article Snippet: .. The recovered PCR products were connected with the linear pIZT-V5/His vector at the Xba I and Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation Article Snippet: .. GFP-Fis1 was produced by PCR-based cloning of rat Fis1 [amplified from p3xFLAG-CMV-10-Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Article Title: SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11 Article Snippet: pRK5-EGFP-Tau (0N4R) was a gift from Karen Ashe (Addgene plasmid # 46904) [ ]. .. To construct pcDNA-FLAG-tau, tau cDNA was amplified by PCR with primers (5′-ACGATGACAAGCTTGGCATGGACGAGCTGTACAAG-3′ and 5′- GATATCTGCAGAATTCTCGACTCACAAACCCTGCTT-3′) and inserted between the Kpn I and Cloning:Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation. Article Snippet: .. GFPFis1 was produced by PCR- based cloning of rat Fis1 [amplified from p3xFLAG- CMV- 10- Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation Article Snippet: .. GFP-Fis1 was produced by PCR-based cloning of rat Fis1 [amplified from p3xFLAG-CMV-10-Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Article Title: SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11 Article Snippet: pRK5-EGFP-Tau (0N4R) was a gift from Karen Ashe (Addgene plasmid # 46904) [ ]. .. To construct pcDNA-FLAG-tau, tau cDNA was amplified by PCR with primers (5′-ACGATGACAAGCTTGGCATGGACGAGCTGTACAAG-3′ and 5′- GATATCTGCAGAATTCTCGACTCACAAACCCTGCTT-3′) and inserted between the Kpn I and Article Title: Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation Article Snippet: .. The coding sequences of Arabidopsis UVR8 wild-type and mutants N-terminally fused with GFP, and RUP1, RUP2 or COP1 N-terminally fused with HA were cloned into the pcDNA3.1 mammalian expression plasmid (ThermoFisher) between the Hin dIII and Amplification:Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation. Article Snippet: .. GFPFis1 was produced by PCR- based cloning of rat Fis1 [amplified from p3xFLAG- CMV- 10- Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation Article Snippet: .. GFP-Fis1 was produced by PCR-based cloning of rat Fis1 [amplified from p3xFLAG-CMV-10-Fis1, a gift from M. Schrader (University of Exeter, UK)] into the Hind III and Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Article Title: SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11 Article Snippet: pRK5-EGFP-Tau (0N4R) was a gift from Karen Ashe (Addgene plasmid # 46904) [ ]. .. To construct pcDNA-FLAG-tau, tau cDNA was amplified by PCR with primers (5′-ACGATGACAAGCTTGGCATGGACGAGCTGTACAAG-3′ and 5′- GATATCTGCAGAATTCTCGACTCACAAACCCTGCTT-3′) and inserted between the Kpn I and Expressing:Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Article Title: Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation Article Snippet: .. The coding sequences of Arabidopsis UVR8 wild-type and mutants N-terminally fused with GFP, and RUP1, RUP2 or COP1 N-terminally fused with HA were cloned into the pcDNA3.1 mammalian expression plasmid (ThermoFisher) between the Hin dIII and Construct:Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Article Title: SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11 Article Snippet: pRK5-EGFP-Tau (0N4R) was a gift from Karen Ashe (Addgene plasmid # 46904) [ ]. .. To construct pcDNA-FLAG-tau, tau cDNA was amplified by PCR with primers (5′-ACGATGACAAGCTTGGCATGGACGAGCTGTACAAG-3′ and 5′- GATATCTGCAGAATTCTCGACTCACAAACCCTGCTT-3′) and inserted between the Kpn I and Synthesized:Article Title: Evolution-guided prioritization identifies a tissue-specific phosphorylation switch on herpes simplex virus 1 UL7 regulating viral replication and pathogenicity Article Snippet: .. pRetroX-TRE3G-UL6o, used to generate stable Vero cells with tetracycline-inducible UL6 expression, was constructed by cloning the entire coding sequences of codon-optimized UL6o amplified via PCR from pUC57-UL6o, which were synthesized (GenScript) using the primers 5′-gcggatccgcaaccatgacagc-3′ and 5′-gcgaattcgcaagagcgacagcgagcg-3′, into Bam HI and Sequencing:Article Title: SUMO1 modification of 0N4R-tau is regulated by PIASx, SENP1, SENP2, and TRIM11 Article Snippet: pRK5-EGFP-Tau (0N4R) was a gift from Karen Ashe (Addgene plasmid # 46904) [ ]. .. To construct pcDNA-FLAG-tau, tau cDNA was amplified by PCR with primers (5′-ACGATGACAAGCTTGGCATGGACGAGCTGTACAAG-3′ and 5′- GATATCTGCAGAATTCTCGACTCACAAACCCTGCTT-3′) and inserted between the Kpn I and Clone Assay:Article Title: Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation Article Snippet: .. The coding sequences of Arabidopsis UVR8 wild-type and mutants N-terminally fused with GFP, and RUP1, RUP2 or COP1 N-terminally fused with HA were cloned into the pcDNA3.1 mammalian expression plasmid (ThermoFisher) between the Hin dIII and Plasmid Preparation:Article Title: Phosphorylation of Arabidopsis UVR8 photoreceptor modulates protein interactions and responses to UV-B radiation Article Snippet: .. The coding sequences of Arabidopsis UVR8 wild-type and mutants N-terminally fused with GFP, and RUP1, RUP2 or COP1 N-terminally fused with HA were cloned into the pcDNA3.1 mammalian expression plasmid (ThermoFisher) between the Hin dIII and |